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建立高效液相色谱法测定武夷岩茶中不同产地、加工工艺过程中茶叶的咖啡因含量,采用Agilent Tc C18柱(4.6 mm×150mm,5μm);流动相:乙腈和水;流速:1.000 mL/min;柱温:30℃;检测波长:274 nm;进样量:10μL进行测定.并讨论了溶剂种类、提取时间、提取功率、净化剂选择等因素对咖啡因的影响.实验结果表明:采用甲醇作为溶剂、超声波时间为25 min,提取功率720 W,采用无水Na_2SO4和大孔树脂作为净化剂为提取茶叶中的咖啡因最佳提取条件,分析8个样品得知在不同产地、加工工艺过程中茶叶中咖啡因成分在含量上具有较大差异,且具有明显的地域特征.以茶叶中咖啡因含量变化为指标,可以作为区分武夷岩茶产地武夷岩茶品质之一的指标.
Abstract：HPLC was established to determine the caffeine content of Wuyi Rock Tea from different origins. The Aguilent TC C18(4.6 mm × 150 mm, 5 μm) was used. The mobile phase included acetonitrile and water, and the flow rate was 1.000 mL/min; the column temperature was 30 ℃; the detection wavelength was 274 nm, and the sample feed was 10 μL for determination. The effects of the solvent type, extraction time, extraction power and purification agent selection on caffeine were discussed. The experimental results showed that methanol was used as a solvent, the ultrasonic time was 25 min, the extraction power was 720 W, and anhydrous Na_2SO4 and macroporous resin were used as purifiers to extract the best conditions for the extraction of caffeine in tea leaves. The analysis of 48 samples showed that there were significant differences in the content of caffeine in teas in different producing areas and processing processes, and there were obvious regional characteristics. With the change of caffeine content in Wuyi Rock Tea as the index, it can be one of the effective factors to distinguish the origin of Wuyi Rock Tea.
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基本信息:
中图分类号:O657.72;TS272.7
引用信息:
[1]金晓怀,林志銮,张传海,等.武夷茶叶咖啡因含量研究及其聚类分析[J].云南民族大学学报(自然科学版),2020,29(04):322-327.
基金信息:
福建省科技厅项目(WYKF2017-8,2018L3018,2017Y0076);; 武夷学院引进人才基金(YT201705)
2020-07-10
2020-07-10