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通过中国知网、PubMed数据库收集三叶青活性成分；借助中药系药理学数据库与分析平台（TCMSP）对活性成分进行搜索并获取其作用靶标；通过在线人类孟德尔遗传数据库（OMIM）、DisGeNET和GeneCards数据库获取病毒性脑膜炎治疗靶点；采用Cytoscape软件构建“药物-成分-靶点-疾病”网络及“成分-靶点-通路”网络；使用R语言进行基因本体论（GeneOntology,GO）功能富集和京都基因与基因组百科全书（Kyoto Encyclopediaof Genes and Genomes, KEGG）通路富集分析.从三叶青中筛选出12个主要活性成分和181个作用靶点；与796个病毒性脑膜炎相关靶点取交集，得到86个药物-疾病共同靶点，蛋白互作网络（protein-protein interaction networks, PPI）中心主要涉及肿瘤蛋白53(tumor protein 53, TP53)、白细胞介素-6(interleukin-6, IL-6)、AKT丝氨酸/苏氨酸激酶1(AKT serine/threonine kinase 1, AKT1)、JUN原癌基因（jun proto-oncogene, JUN）、白细胞介素-1β(interleukin-1 beta, IL-1β)等关键靶蛋白. KEGG富集分析主要涉及AGE-RAGE信号通路、乙型肝炎、TNF信号通路、人类巨细胞病毒感染等相关通路.因此，三叶青防治病毒性脑膜炎可能具有多成分、多靶点、多通路的潜在作用机制.
Abstract：Exploring the mechanism of Tetrastigma hemsleyanum in treating viral meningitis based on network pharmacology and molecular docking technology. Active ingredients of T. hemsleyanum were collected from the China National Knowledge Infrastructure(CNKI) and PubMed databases. The Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP) was utilized to search for these active ingredients and identify their corresponding targets. Therapeutic targets for viral meningitis were obtained from the Online Mendelian Inheritance in Man(OMIM), DisGeNET, and GeneCards databases. Cytoscape software was employed to construct a “drug-component-target-disease” network and a “component-target-pathway” network. Gene Ontology(GO) functional enrichment and kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analyses were performed using R language. A total of 12 major active components and 181 potential targets were screened from T. hemsleyanum. By intersecting these with 796 viral meningitis-related targets, 86common drug-disease targets were identified. The protein-protein interaction(PPI) network highlighted key target proteins such as tumor protein 53(TP53),interleukin-6(IL-6), AKT serine/threonine kinase 1(AKT1), jun proto-oncogene(JUN), and interleukin-1 Beta(IL-1β). KEGG enrichment analysis revealed significant pathways, including the AGE-RAGE signaling pathway, hepatitis B, TNF signaling pathway, and human cytomegalovirus infection. Therefore, T. hemsleyanum may exert its potential therapeutic effects against viral meningitis through a multi-component, multi-target, and multi-pathway mechanism.
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基本信息:
DOI：
中图分类号:R285
引用信息:
[1]孙宗淼,许秀萍.三叶青治疗病毒性脑膜炎的作用机制探索[J].云南民族大学学报(自然科学版),2025,34(03):261-267.
基金信息:
山东省中医药科技项目（Q-2022020）