| 537 | 0 | 25 |
| 下载次数 | 被引频次 | 阅读次数 |
采用十八烷基硅烷键合硅胶为填充剂的色谱柱（250 mm×4.6 mm,5μm），以甲醇和体积分数为0.1%的磷酸水溶液为流动相，建立了高效液相色谱（HPLC）测定雀嘴茶中熊果苷质量分数的方法，该方法在0.349～402.788μg/mL质量浓度范围内具有良好的线性关系，精密度、稳定性、重复性均较好，并优化了提取雀嘴茶中熊果苷的方法.这不仅为雀嘴茶中熊果苷的质量分数测定提供了可靠的分析手段，也为茶叶及其他植物性产品中的活性成分检测提供了新思路.
Abstract：A chromatographic column(250 mm × 4. 6 mm, 5 μm) with octadecylsilane-bonded silica gel as the filler was used, with methanol and a volume fraction of 1% phosphoric acid aqueous solution as the mobile phase, to establish a method for determining the mass fraction of arbutin in tea using high-performance liquid chromatography(HPLC). The method demonstrates a good linear relationship within the mass concentration range of 0. 349 to 402. 788 μg/mL, with satisfactory precision, stability, and repeatability. Furthermore, the extraction method for arbutin in tea was optimized with respect to extraction procedures and solvents. This method not only provides a reliable analytical technique for determining the mass fraction of arbutin in tea but also offers new insights for detecting active ingredients in tea and other plant-based products.
[1]中国科学院中国植物志编辑委员会编.中国植物志，第57(1）卷[M].北京：科学出版社，1999:93.
[2]吴征镒.云南种子植物名录（下册）[M].昆明：云南人民出版社，1984:1138-1145.
[3]国家中医药管理局中华本草编委会.中华本草，第16卷[M].上海：上海科学技术出版社，1999:48-49.
[4]GAO S H,ZHAO T R,LIU Y P,et al.Phenolic constituents,antioxidant activity and neuroprotective effects of ethanol extracts of fruits,leaves and flower buds from Vaccinium dunalianum wight[J].Food Chemistry,2022,374:131752.
[5]WANG Y,TIAN L,WANG Y.Protective effect of Que Zui tea hot-water and aqueous ethanol extract against acetaminopheninduced liver injury in mice via inhibition of oxidative stress,inflammation,and apoptosis[J].Food&Function,2021,12:2468-2480.
[6]ZHANG J K,ZHOU X L,WANG X Q,et al.Que Zui tea ameliorates hepatic lipid accumulation and oxidative stress in high fat diet induced nonalcoholic fatty liver disease[J].Food Research International,2022,156:111196.
[7]王慧琛，王丽琴.熊果苷的应用及检测研究进展[J].天津药学，2012,24(4):71-74.
[8]阎雪莹，王雪莹，张玉华.熊果普研究及应用进展[J].中医药信息，2007,24(4):18-22.
[9]唐婕妤，彭菲.熊果苷的药理作用与资源获取途径研究进展[J].今日药学，2015,25(9):673-677.
基本信息:
DOI：
中图分类号:TS272.7;O657.72
引用信息:
[1]倪芹,王汝雄,李俊璇等.HPLC法测定雀嘴茶中熊果苷的质量分数[J].云南民族大学学报(自然科学版),2025,34(03):277-283.
基金信息:
云南省“生物医药领域”科技计划项目（2019ZF010）