云南民族大学学报(自然科学版)

以扭肚藤总多糖提取率为指标，以超声功率、超声时间(t)、提取温度(T)和液料比为考察因素，采用单因素和响应面实验优化扭肚藤多糖的提取工艺，并考察对脂多糖(LPS)诱导的RAW264.7细胞分泌炎症因子的影响.优化的扭肚藤多糖超声提取最佳工艺为t 33 min，超声功率341 W,T 62℃，液料比39 mL/g，提取率为(6.98±0.34)%.扭肚藤多糖以剂量依赖的方式，抑制LPS诱导的TNF-α、IL-1β和IL-6的生成，促进IL-10的产生.本文建立的扭肚藤多糖超声提取工艺稳定，方法可行；提取得到的扭肚藤多糖具有一定的体外抗炎活性，为扭肚藤的深入研究提供了依据.

To optimize the ultrasonic extraction process of polysaccharides from Jasminum elongatum(Bergius)Willd. and study its anti-inflammatory activity in vitro. The total polysaccharide yield from Jasminum elongatum served as the primary response indicator. Ultrasound power, time, extraction temperature, and liquid-solid ratio were selected as independent variables to optimize the extraction process using both single-factor experiments and response surface methodology(RSM). Additionally, the impact on inflammatory factors released by LPSstimulated RAW264.7 cells was evaluated. The optimal ultrasonic extraction parameters for Jasminum elongatum polysaccharides was obtained as follows: ultrasonic time of 33 min, ultrasonic power of 341 W, extraction temperature of 62 ℃, liquid-solid ratio of 39 mL/g. Under these conditions, the polysaccharide yield reached(6.98 ±0.34)%. The polysaccharides from Jasminum elongatum exhibited a dose-dependent inhibited the LPS-induced production of TNF-α, IL-1β, and IL-6 while enhancing the generation of IL-10. The optimized ultrasonic extraction process of polysaccharides from Jasminum elongatum is stable and feasible. The polysaccharides from Jasminum elongatum demonstrated anti-inflammatory activity in vitro, which provides theoretical basis for the further research on Jasminum elongatum.