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探讨罗布麻叶不同极性溶剂提取物对高脂血症的调节作用及其机制.体内实验表明，罗布麻叶各提取物能显著降低高脂血症大鼠血清总胆固醇(TC)、甘油三酯(TG)和低密度脂蛋白水平(LDL-C)水平，提高高密度脂蛋白水平(HDL-C)水平，并改善肝脏组织病理变化.网络药理学筛选出罗布麻叶与HLP的共同靶点91个，基因本体论(GO)与京都基因与基因组百科全书(KEGG)富集分析提示其作用机制可能与AGE-RAGE、TNF、IL-17、p53和PI3K-AKT等信号通路相关.分子对接验证显示，罗布麻叶活性成分芦丁、异槲皮苷、紫云英苷、白麻苷、槲皮素、金丝桃苷、山奈酚与绿原酸与核心靶点BCL2、AKT1、MMP9、PTGS2、TP53、ESR1具有良好结合潜力.
Abstract：To investigate the regulatory effects and mechanisms of extracts from Apocynum venetum L. leaves using solvents of different polarities on hyperlipidemia. In vivo experiments showed that all extracts of Apocynum venetum L. leaves significantly reduced the serum levels of total cholesterol(TC), triglycerides(TG), and low-density lipoprotein cholesterol(LDL-C) in hyperlipidemic rats, increased the level of high-density lipoprotein cholesterol(HDL-C), and improved the pathological changes of liver tissue. Network pharmacology identified 91 common targets between Apocynum venetum L. leaves and hyperlipidemia(HLP). Gene Ontology(GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) enrichment analyses suggested that its mechanism of action may be related to signaling pathways such as AGE-RAGE, TNF, IL-17, p53, and PI3K-AKT. Molecular docking verification indicated that the active components of Apocynum venetum L. leaves, including rutin, isoquercitrin,astragalin, cynaroside, quercetin, hyperoside, kaempferol, and chlorogenic acid, have good binding potential with the core targets BCL2, AKT1, MMP9, PTGS2, TP53, and ESR1.
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基本信息:
中图分类号:R285.5
引用信息:
[1]靳雅,张荣,王洋洋,等.网络药理学结合实验探讨罗布麻叶降脂机制[J].云南民族大学学报(自然科学版),2026,35(01):8-16.
基金信息:
新疆维吾尔自治区地州科学基金（2022D01F33）; 新疆维吾尔自治区高校科研计划（XJEDU2022P148）; 新教函[2024]167号“天山英才”培养计划教育教学名师培养项目; 新疆维吾尔自治区自然科学基金青年科学基金（2022D01B152）